Experimental and observational data suggest that intestinal inflammation arises from abnormal immune response to intestinal microbiota in genetically susceptible individuals. Not all subjects who carry common risk alleles develop the disease suggesting that environmental triggers are important
in disease expression.We hypothesis that norovirus may play a role in the pathogenesis of Crohn’s disease. In an observational pilot study, we aimed to determine the prevalence of norovirus in Crohn’s disease and ulcerative colitis subjects compared with controls. Methods: Stool samples were collected from consecutive subjects with confirmed Crohn’s disease (CD), ulcerative this website colitis (UC) and controls. Viral RNA was extracted and human norovirus detection was performed using real time polymerase chain reaction. Results: A total of 102 subjects with inflammatory bowel diseases (IBD) (61 CD; 41 UC; 19 active disease) and 102 healthy controls were included. All stool samples from cases and controls were tested negative for the norovirus genogroup II, the most prevalent group of norovirus circulating locally and elsewhere. Conclusion: Human norovirus is absent in the stool of subjects with IBD (active and inactive) and controls. Norovirus may only play a minor role, if any,
in pathogenesis or disease Regorafenib in vivo flare in IBD. Key Word(s): 1. IBD; 2. Norovirus; 3. Crohn’s disease; Presenting Author: YAO HE Additional Authors: SHUNHUA LONG, MINHU CHEN, KANG CAO, YUJUN CHEN, BAILI CHEN, REN MAO, SHENGHONG ZHANG, ZHIRONG ZENG, PINJIN HU Corresponding Author: MINHU CHEN, PINJIN HU Affiliations: The First Affiliated MCE Hospital of Sun Yat-Sen University; The First Affiliated Hospital of Nanchang University; The Sixth Affiliated Hospital of Sun Yat-Sen University Objective: The purpose of this study was to investigate the role of the PI3K/Akt/mTOR
signaling pathway and it’s negative feedback factor PTEN in the pathogenesis of Crohn’s disease(CD). Methods: Peripheral blood was collected from 24 patients with CD (n = 24) and 16 healthy donors (Control,n = 16). Tissue samples were obtained endoscopically from patients with CD as study group (n = 23) and patients with constipation as control (n = 12). Changes of PI3K/Akt/mTOR pathway and PTEN expression were evaluated by quantitative real time PCR(qPCR) and western blotting respectively in peripheral CD4+ T Cells, and by a standard immunohistochemical procedure in mucosal lymphocytes. Results: No significant difference of mRNA expression of PI3K, Akt, mTOR, 4E-BP1, P70 S6K was found in peripheral CD4+ T Cells between CD patients and control, though a tendency of up-regulation of these mRNA expressions in CD patients was noted.