Age and sex matched control group (18 volunteers) was also included into the study. PCBs were determined in blood samples by capillary gas chromatography with the electron capture detector (column SE-54, internal standard PCB119). Identification of individual congeners was carried out by a relative retention times, quantitative calculations were performed using relative response factors. Results: Mean serum total PCBs concentration was significantly higher in NASH patients Dorsomorphin cell line in compare to healthy controls (1,46 ± 1,39 vs 0,66 ± 0,29 ng/g, p=0,02). There were no differences between groups in serum concentrations of congener 118 (0,22 ±0,14 vs 0,20 ±0,16 ng/g p=0,2). Congeners

183 and 185 were found only in 41% of controls, but not in patients with NASH. Mean serum concentration of congeners 99, 101, 138 and 153 were lower in control in compare to patients with NASH (0,12 ± 0.05 vs 0.06 ± 0.04, p=0.007; 0.72 ± 0.57 vs 0.05 ± 0.07, p=0.0002; 0.48 ± 0.78 vs 0.08 ± 0.06, p=0.04; 0.33 ± 0.33 vs 0.06 ± 0.07, p=0.007). In conclusion, total serum PCBs concentration and concentration of congeners 99, 101, 138 and 153 were lower in healthy control than in patients with NASH. Congeners 183 and 185 were found only in healthy controls. It means that NASH patients in compare to healthy controls

experienced long-term exposure for toxic lipophilic environmental pollutants, which can be additional factors facilitating development and progression of NAFLD. Further studies are needed to clarify Cobimetinib price the role of different congeners and its transporters 上海皓元 in liver for development of the disease. Disclosures: Vasily Isakov – Advisory Committees or Review Panels: Abbvie, Bristol-Myers Squibb, Gilead, Janssen, Merck,

Vertex; Consulting: Bristol-Myers Squibb, Merck; Speaking and Teaching: Bristol-Myers Squibb, Janssen, Merck The following people have nothing to disclose: Vladimir Bessonov, Elena Khromchenkova, Natalia Topilskaya, Ksenia Selezneva, Victor Tutelyan Background: AgRP is an orexigenic peptide directly regulated by fatty acid uptake; FASN is involved in fatty acid synthesis and its expression is sensitive to glucose concentration. Aim: The aim of this pilot study is to measure the expression of AgRP and FASN in vitro under elevated glucose and lipid concentrations and compare findings to a set of NAFLD patients. Methods: HepG2 cells were challenged with 10mM of oleic acid (OA), 50mM glucose (GLU), or both and expression of AgRP and FASN was measured by qPCR and compared to untreated controls. Visceral adipose tissue was collected and flash frozen for preconcented patients with biopsy-proven NAFLD and AgRP and FASN gene expression was measured by qPCR. Additionally, expression of both genes was measured in formalin fixed paraffin embedded hepatic tissue from a subset of patients.