Disruption of the epithelial barrier Trametinib clinical trial is often associated with the increase of cell shedding. This study aims to investigate the mechanisms how mucosal protectants maintain the integrity of intestinal epithelial barrier in

indomethacin-induced enteropathy by observing real-timely the gap density using confocal laser endomicroscopy (CLE). Methods: A method to evaluate real-timely the intestinal epithelial barrier damage after administration of indomethacin in rats were established using a new technique CLE by investigating the gap density in small intestine. Then the mucosal protectant teprenone and proton pump inhibitor rabeprazole were given by gavage before and after the administration of indomethacin. Then, the mechanisms of how these medicines affect the intestinal epithelial barrier were investigated by investigating gap density and TNF- α pathway. Results: Gaps could be clearly observed by CLE, Gap density increased after indomethacin administration. During this process, the expressions of TNF- α, NF-kB and Caspase-3 were up-regulated while the expressions of tight junction were down-regulated. Teprenone and rabeprazole could interfere in the damage process and protect the integrity of the epithelial

barrier. Conclusion: CLE can be more objective, accurate and real-time to selleckchem investigate gap density. Teprenone and rabeprazole could prevent indomethacin-induced intestinal demage and improve the integrity of the epithelial barrier mediated by the intervention of TNF-α pathway. Key Word(s): 1. Epithelial barrier; 2. endomicroscopy; 3. Gap density; 4. TNF- α; Presenting Author: YING KIT LEUNG Corresponding Author: YING KIT LEUNG Affiliations: Precious Blood Hospital Objective: We have previously demonstrated that the vasculature in villi are of the reverse fountain pattern, one-uo, one down pattern or the reticular pattern. Whether these pertain to a arteriole-venule pattern

or mainly comprise of capillaries is not yet confirmed Verteporfin scientifically. The aim of this study is to visualize how blood elements flow in the vasculature of the villi in a living human under sedation, hence infer the basic characteristic of such blood vessels. Methods: Ten subjects underwent colonoscopy and probe-based confocal laser endomicroscopy (pCLE) were included into the study. The indications of the procedure being: inflammatory bowel disease, familial adenomatous polyposis, colonic polyps and abdominal pain. Fluorescein was injected after the small intestine was entered, and the villi examined with pCLE which took video images at 12/sec. The velocities of cellular movement in the vessels were determined during playback of the videos, and compared with similar measurement of flow around colonic crypts. Results: Blood elements of diameter around 7–10 microns, move in the vessels in an episodic manner.