g., T. bryantii of ruminants, T. primitia from termites), pathogens (T. pallidum spp.) or as part of a pathogenic complex of bacteria (T. denticola, T. vincentii, and others from the oral cavity) [20, 22]. Additionally, several different phylogenetic groups of Treponema species have been isolated or identified in digital dermatitis lesions, with

similarities to T. denticola, T. phagedenis, T. vincentii, T. medium, and the proposed new species T. brennaborense and T. pedis[16, 23–27]. Four Treponema spirochetes were isolated Selleckchem Fedratinib from DD lesions on an Iowa dairy, and the characterization presented here demonstrates that they are highly similar to the T. phagedenis type strain. Despite classification as the same genus, these organisms occupy not just different hosts (bovine vs. human), but also very different anatomical locations (dermis adjacent to heel bulb and dewclaw vs. genitalia). There most likely exists some overlap of microenvironment within these anatomical locations (low oxygen availability,

epithelial cell layers, etc.) as both the DD isolates and T. phagedenis have similar growth characteristics and nutrient requirements. Other pathogenic organisms such as Mycobacterium intracellulare, Yersinia species and Bacillus species have identical 16 s rRNA gene sequences and are highly genetically similar based on DNA-DNA hybridization [28]. However, they exhibit distinct “ecophysiological” properties based on virulence phenotypes or host ranges. Some are distinct species, Y. pestis and Y. pseduotuberculosis for example, this website while others are merely different serovars within the species, such as M. intracellulare. Some pathogens are separated from other genetically identical species by acquisition of a plasmid conferring pathogenic properties. Evaluation of the draft contigs of T. phagedenis and the DD isolates do not give any

indication of acquisition of a plasmid that would have conferred the expansion of host range or conversion into a more virulent organism. These studies herein led us to develop a growth medium reduced in complexity so that the individual nutrients and growth factors of previously isolated spirochetes could be further evaluated. While the list of components appear similar to fastidious anaerobe broth used by many groups [17, 29], the quantities of several components are ZD1839 chemical structure greatly reduced. Systematic studies on essential nutrients and environmental growth factors of the non-pallidum treponemes are scarce [22] and consist of a few incomplete lists in such reference texts as Bergey’s Manual of Systematic Bacteriology and The Prokaryotes [18, 21]. A recently published report showed that isolate 1A achieved log phase growth in 3 to 5 days of culture in a rich media similar to fastidious anaerobe broth [29] consistent with our results in both media types. We have defined temperature tolerances, pH tolerances and essential growth CRT0066101 mouse requirements (serum and VFAs) of isolate 4A.