In order to make those measurements, four samples per plot, at a distance of 0.10m from Volasertib aml the plant stem, between 6:30 and 7:30 a.m., were taken. These samples were mixed to make a composed sample. The analyses were made using the 1:1.5 extraction method [15]. Leaf area was measured at the end of the cycle (89 DAT) by measuring the width of all leaves of the plant and making use of the mathematical model (AF = 0.826L1.89 (R2 = 0.97)) to calculate it [16]. First and second fruits had their weight determined and also total yield in kilograms of fruits per plant. The data were submitted to the analysis of variance by the F test and the polynomial regression analysis. The data related to the first and second fruits weight and the total yield per plant were analyzed by a regression study by the response surface methodology analysis.

3. Results and Discussion Measurement made 46 DAT showed that leaf N content was significantly influenced only by the N concentration in the nutrients solution (Table 2) with the means showing adjustment to a first-degree equation (Figure 1). An increment of 30% in the N level (39.1 and 50.8gkg?1 of N) was verified when the nutrients solution used to fertigate the plants had the smallest and the highest N concentration, respectively.Figure 1N content in the leaf used for the evaluation of the plant nutritional status 46 days after transplantation (DAT) (Y1) and leaf area 89 DAT (Y2) as influenced by the concentrations of N in the nutrients solution.

Table 2Analysis of variance results for nitrogen leaf level (LN) and potassium leaf level (LK), N-NO3?, hydrogen ion potential (pH), electrical conductivity (CE), and N (NS) and K (KS) concentrations in the solution of substratum.The K leaf content was influenced by the interaction of the factors (Table 2). Significant adjustments to first- and second-degree equations were verified in accordance with the N and K combination (Figure 2). While in the nutrients solution of 4mmolL?1 of K the increase in N concentration resulted in a reduction in the level of K in the leaves. In the concentrations of 6, 8, and 10mmolL?1 of K, no significant adjustment to a polynomial equation was observed and resulted in the levels of 31.9, 34.4, and 37.2gkg?1 of K in the leaves, respectively (Figure 2(a)). On the other hand, in all N concentrations increments in K concentration were verified Dacomitinib with each increment in K concentration, and the highest levels were observed when the nutrients solution had the lowest concentration of N (Figure 2(b)), this being explained by the lower growth of leaf area with the lowest concentrations of N (Figure 1).