9 marker genes for glucosinolate metabolism that have been differentially expressed in our former microarray review were found to possess identical expression patterns in many tag profiling datasets, These involve the ESM1 gene, a marker for isothiocyanate production which was inferred to be up regulated in P. fastigiatum in all ten datasets. The MVP1 gene a myrosinase connected protein exclusively interacting with TGG2, was also up regulated in P. fastigiatum in all datasets except A0 and AL0. The ESP gene, a marker for nitrile production at the same time as two marker genes for the production of methionine derived glucosinolates with 4 carbon atoms had been up regulated in P. enysii in all datasets ex cept A0 and AL0 and two other marker genes for C4 glu cosinolates had been up regulated in P.
enysii in all datasets. AT1G74100 was up regulated in P. enysii while in the PL0 dataset and AT4G03060 was up regulated in P. enysii while in the AL1 and AL2 more bonuses datasets. Each microarray analyses and tag profiling identified dif ferentially expressed glucosinolate metabolism genes not observed using the other process, With tag profil ing five extra genes had been inferred for being up regulated in P. fastigiatum, AT1G62540, AT4G13770, AT5G23020, AT5G60890, With tag profiling twelve added genes had been inferred to get up regulated in P. enysii, AT1G62560, AT2G20610, AT3G19710, AT5G26000, AT5G48180, AT5G61420, AT5G07690, AT5G25980, AT5G61210, AT1G04750, AT1G59870, The homologs to AT1G54000, AT1G31180, AT2G14750, AT3G58990, and AT4G03050 were up regulated in P. eny sii in the microarray examination only.
Contradictory final results had been obtained for that homologue to AT1G54020, read more here a myrosinase related protein closely connected to ESM1 and MVP1, since it was up regulated in P. fastigiatum during the micro array evaluation but in P. enysii when measured by tag profiling. Response to cold Populations of P. fastigiatum expand at a imply altitude of 1,485 m, even though P. enysii grows between heights of 1,476 and two,492 m, Both plants, but P. enysii considerably much more so, are subject to cold temperatures. An enrichment of GO terms corresponding to cold stimulus was detected during the microarray experiment for P. fastigiatum, Although this GO phrase was not enriched with tag profiling, two genes associated with cold tolerance in the. thaliana and AT1G20440 have been up regulated in P. fastigiatum in all tag profiling datasets. Up regulation of AT1G20440 was also detected in P. fastigia tum with the microarray. Other genes relevant to cold toler ance and inferred to be up regulated in many, but not all tag profiling datasets have been AT1G20450, AT1G04400, AT2G45660, AT4G22950, AT2G33835, and AT4G25140 in P.