With regard to the RG-7388 in vivo targeting ability of BSA-Au-FA, we evaluated the cellular selective uptake of BSA-Au-FA with a MGC803 MK5108 in vivo cell in an RPMI-1640 medium without FA, which was carried out and contrasted with the other two groups: (a) cells treated with BSA-Au in RPMI-1640 medium without FA and (b) cells treated with BSA-Au-FA in RPMI-1640
medium with FA. After a 30-min incubation, only the cells incubated with BSA-Au-FA in RPMI-1640 medium without FA displayed abundant golden dots (Figure 6c) and a red fluorescence signal (Figure 6d) on the membrane of the cells, indicating selective targeting of nanocomplexes on MGC803 cells. Figure 6 Dark-field scattering images (a, c) and fluorescence images (b, d). (a, b) Low-magnification image of targeted MGC803 cells incubated with 50 μg/mL of buy Givinostat BSA-Au nanocomplexes for 2 h. (c, d) High-magnification image of targeted MGC803 cells incubated with 50 μg/mL of
BSA-Au nanocomplexes for 30 min, monitored by dark-field and fluorescence microscopy. Conclusion In summary, biocompatible BSA-Au nanocomplexes were successfully synthesized in water at room temperature by a protein-directed, solution-phase, green synthesis method. The as-prepared BSA-Au nanocomplexes showed highly selective targeting and dark-field and fluorescence imaging on MGC803 cells. It may have great potential in applications such as tumor targeting imaging, drug delivery, and ultrasensitive detection. The current study provides further evidence of the biomimetic fabrication of functional materials and exemplifies
the interactions between PAK6 proteins and metal nanomaterials in an attempt to create novel bioconjugated composites. Acknowledgment This work is supported by the National Key Basic Research Program (973 Project) (2010CB933901 and 2011CB933100), National 863 Hi-tech Project of China (no. F2007AA022004), Important National Science & Technology Specific Projects (2009ZX10004-311), National Natural Scientific Fund (81225010, 1101169, 31100717, 81272987, 51102258), New Century Excellent Talent of Ministry of Education of China (NCET-08-0350), and Zhejiang Provincial Natural Science Foundation of China (LY12H11011). Electronic supplementary material Additional file 1: Supporting information. A document showing two supplementary figures: the TEM image of BSA-Au nanocomplexes in long aging time and the FT-IR spectra of (a) BSA and (b) BSA-Au nanocomplexes. (DOC 286 KB) References 1. Gao X, Cui Y, Levenson RM, Chung LWK, Nie S: In vivo cancer targeting and imaging with semiconductor quantum dots. Nat Biotechnol 2004, 22:969–976.CrossRef 2. Basabe-Desmonts L, Reinhoudt DN, Crego-Calama M: Design of fluorescent materials for chemical sensing. Chem Soc Rev 2007, 36:993–1017.CrossRef 3. Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML, Lukyanov SA: Fluorescent proteins from nonbioluminescent Anthozoa species. Nat Biotechnol 1999, 17:969–973.CrossRef 4.