In the laboratory, serum was separated and analyzed for albumin, retinol, and zinc concentration. Biochemical analysis Hb concentration and WBCs were measured directly using an automatic analyzer (Sysmex Microdilutor F-800, Kobe, Japan). ESR was determined directly using the Westergreen technique.[18] Serum Albumin was determined by the Pacritinib clinical trial bromcresol green method.[19] Inhibitors,Modulators,Libraries Serum retinol was measured using the Retinol Binding Protein (human) enzyme-linked immunosorbent assay kit (Cat. No. AG-45A-0011EK-KI01) and zinc concentration was measured using the simple colorimetric method.[20] Ethical considerations The study was approved by the Institutional Ethics Committee of King George’s Medical University, Uttar Pradesh, Lucknow, India. Informed consent was obtained from each subject before the start of the study.

Statistical analysis Inhibitors,Modulators,Libraries The data collected were entered in Microsoft Excel sheet and checked for any inconsistency. The results are presented in mean (��SD) and percentages. The unpaired t-test is used to compare the differences Inhibitors,Modulators,Libraries in vitamin A levels between male/female and married/unmarried patients. The one-way analysis of variance is used to compare the vitamin A levels among different age groups. The 95% confidence interval (CI) of means is also calculated and presented. The Pearson correlation analysis is carried out to find out the correlation between vitamin A levels and serum zinc, Hb, serum albumin, WBC, and ESR. The multivariate linear regression is being carried out to assess the effect of serum zinc level to adjust confounding factors such as age, sex, and body mass Inhibitors,Modulators,Libraries index (BMI) of the patients on vitamin A levels.

The P < 0.05 is being Inhibitors,Modulators,Libraries considered as significant. All the analysis is being carried out by using the SPSS 15.0 version. RESULTS A total of 208 patients of TB were studied to determine the serum zinc levels and its association with Vitamin A levels. The background characteristics of the patients are given in Table 1. The mean age of the patients was 30.56 (��11.38) years with range 18-55 years. More than half (54.3%) of the patients were males and 63% of the patients were married. BMI of the patients was 18.40 �� 3.10. The serum zinc and vitamin A levels among the patients were 9.60 (��0.86) ��mol/l and 0.77 (��0.22) ��mol/l respectively. However, Hb, WBC, ESR, and serum albumin were 10.02 (��1.33) g/dl, 10076.01 (��1822.

67) cell/mm3, 14.50 (��2.95) mm/h and 3.40 (��0.32) g/dl Cilengitide respectively. Table 1 Background characteristics of the patients There was a strong correlation between serum zinc and vitamin A levels (r = 0.86, P < 0.01) [Figure 1]. Hb (r = 0.61, P < 0.01) and serum albumin levels (r = 0.87, P < 0.01) were also strongly correlated with the vitamin A levels; however, WBC (r = ?0.60, P < 0.01) and ESR (r = ?0.79, P < 0.01) were negatively correlated with the vitamin A levels [Table 2].