In the same session, adolescents completed the 8 repetitions maximum (RM) loads for each exercise and then, after 72 hours, the 8RM tests were repeated to determine test-retest reliability. In these testing sessions, participants were also familiarized with the OMNI-RES selleck scale. In the following week, the subjects participated in the experimental protocol in randomized order with an interval of 72 hours between exercise sequences. Exercise sessions Two different exercise sequences were designed and composed of alternate lower and upper-body RT exercises (SEQA: ILP, DL, BP and TE) or of two exercises in sequence for the same muscle group (SEQB: ILP, BP, DL and TE). Participants performed the A or B sequence, through a randomized crossover design, at the same time of the day.

Seven subjects performed SEQA first, while the remaining six subjects performed SEQB. The warm-up before each sequence consisted of 12 repetitions of each exercise, in the assigned sequence, with a 20% load of 8RM. After a three minute rest interval, adolescents performed the exercise sequence with 80% of the 8RM and with a 60-bpm cadence (rate of 30 exercise repetitions per minute). RT exercises were performed until concentric failure with a resting period of 90 seconds between exercises. Immediately after each exercise, participants reported their RPE with emphasis on local fatigue (predominantly active muscle groups). After 72 hours, all participants performed the other sequence which they were previously assigned. The procedures and instructions of the first session were maintained in the second exercise session.

Statistical Analyses Descriptive statistics of data were presented as mean (M) and standard deviation (SD). The normality test of Shapiro-Wilk and the homogeneity of variance and covariance were confirmed using the Levene��s test and Mauchly sphericity test. All variables presented normal distribution. Test-retest reliability was examined by using the intraclass correlation (ICC). To compare the number of repetitions performed to failure and RPE in the two sequences, one-way ANOVAs with repeated measures were used followed by post-hoc tests with Bonferroni adjustment for multiple comparisons. Paired t-tests were used to examine specific exercise differences across different sequences. The significance level was set at p<0.05.

Results Initial repeated measures ANOVA included the sexual maturity status as a covariate, but results showed no significant Entinostat effects on the number of repetitions (p=0.083) and on the RPE (p=0.250). Therefore, it was excluded from further analyses. Table 1 presents the number of exercise repetitions performed to failure in both sequences. Table 1 Number of repetitions per exercise in both exercise sequences* Within-subjects analysis showed significant differences in the number of repetitions performed to failure in SEQA (F(3,36)=9.35, p<0.001) and SEQB (F(3,36)=7.22, p=0.001).