In the tumor tissue, Yip1 interacting factor homolog A (S. cerevisiae) (Yif1a) was hypermethylated. Furthermore, selleck chem Rucaparib unambiguous new methylations in the precancerous tissue were branched chain aminotransferase 2, mitochondrial (Bcat2), ubiquinol-cytochrome c reductase (6.4 kDa) subunit (Uqcr), zinc finger and BTB domain containing eight opposite strand (Zbtb8os), chimerin (chimaerin) 2 (Chn2), RIKEN cDNA 1520401A03 gene (1520401A03Rik), sine oculis-related homeobox 3 homolog (Drosophila) (Six3), and WSC domain containing 1, 407 bp (Wscd1).

Newly methylated RAMs in the tumor tissue included chimerin (chimaerin) 2 (Chn2), RIKEN cDNA 1520401A03 gene (1520401A03Rik), sine oculis-related homeobox 3 homolog (Drosophila) (Six3), A disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 17 (Adamts17), annexin A2 (Anxa2), BTB (POZ) domain containing 11 (Btbd11), chemokine (C-C- motif) receptor 4 (Ccr4), calsyntenin 2 (Clstn2), folliculin (Flcn), glutamate decarboxylase-like 1 (Gadl1), Hect domain and RLD 3 (Herc3), like-glycosyltransferase (Large), leucine rich repeat and Ig domain containing 2 (Lingo2), src-related kinase lacking C- and N-terminal myristylation sites (Srms), transcription factor 4 (Tcf4), tubulin tyrosine ligase-like family, member 9, 208 bp (Ttll9), WD repeat domain 17 (Wdr17), zinc finger and SCAN domain containing 22 (Zscan22) and RIKEN cDNA A430078G23 gene (A430078G23Rik). There were no unambiguous hypermethylated or newly methylated RAMs that carried forward from the precancerous to the tumor tissue.

As described in the methods, some genes and genomic regions, which are greater than 10 kb away from a known gene, are tentatively classified as having multiple methylation statuses in either the precancerous or tumor tissue (21%, 16 of the 75 total genes and genomic regions). Table 1 indicates Anacetrapib the methylation statuses and particular treatment periods at which these genes displayed altered methylation. The genes were: DNA segment, Chr 13, Wayne State University 177, expressed (D13Wsu177e), HIG1 domain family, member 2A (Higd2a), protein tyrosine phosphatase, receptor type O (Ptpro), dipeptidylpeptidase 10 (Dpp10), tyrosine kinase nonreceptor 2 (Tnk2), ephrin B2 (Efnb2), predicted gene, “type”:”entrez-nucleotide”,”attrs”:”text”:”EG622408″,”term_id”:”116863828″EG622408 (“type”:”entrez-nucleotide”,”attrs”:”text”:”EG622408″,”term_id”:”116863828″EG622408), prickle-like 2 (Drosophila) (Prickle2), triple functional domain, PTPRF interacting (Trio), transmembrane protein 132d (Tmem132d), and WSC domain containing 1, 359�C361 bp (Wscd1). There were three PCR products which each associated with multiple ��hits�� that displayed the highest BLAT scores (i.e.