In February 2011, the PubMed database was searched for studies of

In February 2011, the PubMed database was searched for studies of HIV testing in community settings conducted in resource-rich countries, after the introduction of highly active antiretroviral therapy (post-1996). Broad search terms were used to maximize the number of results: HIV; testing; screening; community; outreach; voluntary counselling; venues; nonclinical; nonhealthcare; mobile health clinics; community health centres; and needle-exchange

PLX3397 supplier programmes were used in various combinations. Where possible, medical sub-heading (MESH) terms were included in the search. Reference lists of those papers retrieved from the electronic search were reviewed for additional pertinent references. Community HIV testing facilities were defined as those that are

based outside pre-existing traditional healthcare settings. These include both stand-alone HIV testing services, provided separately from other clinical services, and venues primarily used for other purposes (such as social venues or community centres) where HIV testing is available as an additional service. For the purposes of this review, established HIV testing provision within hospitals, primary care facilities, antenatal clinics and sexually transmitted infection (STI) clinics was excluded. Studies were included in the final analysis if they were conducted in a community setting, as defined above, and reported at least one of the following outcome measures: uptake of HIV testing in community settings; HIV seropositivity of populations tested in community settings; client attitudes Selleck Ku-0059436 towards HIV testing in community settings;

or provider attitudes towards HIV testing in community settings. We included studies conducted in resource-rich settings in Western Europe, North America and the Antipodes which were published from 1996 onwards. A total of 3107 papers were identified using the search strategy. Titles, abstracts and full papers were screened independently by two researchers and results from screening by each researcher were compared. After this process, 48 papers were found to contain at least one of the outcome measures of interest and were therefore considered appropriate for data extraction (Fig. 1). These 48 papers oxyclozanide were examined for evidence of duplication of data and four papers were excluded on this basis, giving a final total of 44 papers being included in the review (Table 1). Where papers reported on different outcome measures from the same location, both papers were included in the final analysis. Studies were stratified by the target population and the setting where HIV testing took place. Acceptability of the HIV testing strategy was examined using uptake of testing and client and staff attitudes to testing. Effectiveness of HIV testing was examined with regard to new diagnoses made and transfer of those individuals to appropriate HIV-related care and support services.

A linear gradient of acetonitrile (20–100%) at a flow rate of 1 m

A linear gradient of acetonitrile (20–100%) at a flow rate of 1 mL min−1 was used. Solvent A was deionized water +0.1% trifluoroacetic acid (TFA) and solvent B was acetonitrile +0.1% TFA. Absorbance was monitored at 215 nm. Temperature stability was evaluated by incubating the purified compounds at various temperatures

from 30 to 100 °C for 30 min or at 121 °C for 20 min. Residual anti-Candida activity was determined by disk diffusion assay against C. albicans. The effect of pH was determined using a pH range from 2 to 10 adjusted with diluted HCl or NaOH. After incubation for 2 h at room temperature MDV3100 and neutralization to pH 7, the residual activity was measured. Resistance to proteases was tested by incubating the purified compounds with proteinase K, trypsin, α-chymotrypsin and lipase A at 1 : 10 or 1 : 5 (w/w) ratios, as described previously (Tabbene et al., 2009). HPLC-purified fractions were subjected to TLC using n-butanol–methanol–water (39 : 10 : 20, v/v/v) buy Vincristine as the mobile phase. The bioassay was performed using C. albicans ATCC 10231. TLC plates

were sprayed with water for the detection of hydrophilic compounds. Spraying with ninhydrin or 4,4′-Bis(dimethylamino)diphenylmethane (TDM) allowed the detection of compounds with free amino groups or with peptide bonds, respectively (Yu et al., 2002) and spraying with Pauly reagent allowed the detection of tyrosine-containing peptides (Jutisz, 1960). Lipopeptide compounds were hydrolyzed in sealed tubes with 6 N HCl at 150 °C for 8 h. After total hydrolysis, liposoluble moieties were extracted with chloroform (Besson et al., 1976), analyzed by TLC

on a silica gel 60 plate in chloroform–methanol–water (65 : 25 : 4) and revealed 3-mercaptopyruvate sulfurtransferase with ninhydrin as described previously (Russell, 1960). HPLC-purified fractions were analyzed using matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF/MS). Samples dissolved in methanol (1 μL) were mixed with 1 μL of matrix (α-cyano-4-hydroxycinnamic acid) at 5 mg mL−1 in 50 : 50 H2O : CH3CN containing 0.1% TFA. Spectra were acquired using a prOTOF 2000 system (Perkin Elmer) operating in positive reflectron mode with an accelerating voltage of 16 kV. The MIC of the purified compounds against human isolates of the pathogen C. albicans was determined by the microbroth dilution assay. One million cells mL−1 of C. albicans were tested for their sensitivity to twofold increasing concentrations of the compounds (from 1.95 to 1000 μg mL−1). Amphotericin B was used as a positive control. After incubation at 28 °C for 24 h, yeast growth was determined by measuring the OD600 nm with a microplate reader (Bioteck, ELx 800). The MIC was defined as the lowest concentration of the compounds inhibiting the yeast growth. MFC was determined from the same experiments by removing 10 μL from wells displaying no yeast growth after 48 h of incubation. Aliquots were spread onto Sabouraud dextrose agar plates, incubated at 28 °C for 24 h and counted.

coelicolor (Yang et al, 2006) The specificity for dCMP incorpor

coelicolor (Yang et al., 2006). The specificity for dCMP incorporation into pORF102 leaves the possibility that either the

SD-208 in vivo first or the second nucleotide of the 3′-end of pAL1 (… GCAGG-3′) may serve as a template for the deoxynucleotidylation reaction. In this study, we identified the gene product of pAL1.102 as a protein that is associated with both termini of the linear Arthrobacter plasmid pAL1. The proposed TP – at least when fused to MBP to ensure solubility – was not capable of specifically recognizing telomeric pAL1 DNA in vitro. However, in an in vitro deoxynucleotidylation assay, the pORF102 protein specifically incorporated dCMP, complementary to the 3′-ends of pAL1. This is consistent with its presumed role as a protein primer in DNA replication. The financial support of the Deutsche Forschungsgemeinschaft is gratefully acknowledged (FE 383/11). We thank Prof. Dr R. Brandsch (University of Freiburg, Germany) for kindly providing the vector pART2, and Prof. Dr A. Steinbüchel (Münster) for access to the phosphoimager. We also thank Manuel Tomm for initial EMSA experiments, and Gabriele Niester and Almut Kappius for technical assistance. Table S1. Primers and ssDNA template

LBH589 purchase used in this study. Please note: Wiley-Blackwell is not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article. “
“Many bacteria produce siderophores for sequestration of growth-essential iron. Analysis of the Salinispora genomes suggests that these

marine actinomycetes support multiple hydroxamate- and phenolate-type siderophore pathways. We isolated and characterized desferrioxamines (DFOs) B and E from all three recognized Salinispora species and linked their biosyntheses in S. tropica CNB-440 and S. arenicola CNS-205 to the des locus through PCR-directed mutagenesis. Gene inactivation of the predicted iron-chelator Cyclooxygenase (COX) biosynthetic loci sid2-4 did not abolish siderophore chemistry. Additionally, these pathways could not restore the native growth characteristics of the des mutants in iron-limited media, although differential iron-dependent regulation was observed for the yersiniabactin-like sid2 pathway. Consequently, this study indicates that DFOs are the primary siderophores in laboratory cultures of Salinispora. Siderophores are small molecules secreted by bacteria to sequester growth-essential ferric iron that is poorly soluble under neutral pH and aerobic conditions (Neilands, 1995). The structures of siderophores vary considerably and are often suited to the environmental niche of the producing bacterium. For example, amphiphilic siderophores possess hydrophobic fatty acid chains that enable them to remain associated with the cell membrane (Martinez et al., 2003) – an attribute particularly advantageous in pelagic marine environments where dilution occurs rapidly.

For comparison, the peak number of providers prescribing lopinavi

For comparison, the peak number of providers prescribing lopinavir/ritonavir occurred in the 18th quarter, with 1288 of 3861 providers (33.4%) prescribing Inhibitor Library lopinavir/ritonavir. Of the 128 facilities prescribing any antiretrovirals within the VHA, the percentage where each target medication had been prescribed rose quickly over the first five-to-six quarters and then rose gradually over the remaining quarters (Fig. 5). The extent of penetration, however, differed markedly among the

four target medications. By quarter 6, atazanavir had been prescribed at 80% of facilities, closely matching the 83% penetration of lopinavir/ritonavir; darunavir and tipranavir had been prescribed at 65% and 56% of facilities, respectively. By the last quarter of the evaluation period atazanavir and lopinavir/ritonavir had been prescribed at over 95% of all facilities. Similar to overall prescribing of antiretrovirals, Pirfenidone price prescribing of the target medications was greatest at facilities with medium-size HIV practices (Fig. 6). Less than 10% of new prescriptions for target medications in each period occurred at facilities with smaller HIV practice sizes. Prescribing at facilities with large and very large HIV practices was similar to prescribing of all other antiretrovirals. Identification of

whether significant variation in new medication uptake exists across a healthcare system may be important, as such variation may reflect differential patient access to new treatment. Uptake of new antiretrovirals in the VHA generally reflected overall prescribing of all antiretrovirals, suggesting that availability and tuclazepam prescribing of these new agents are consistent across the system. Atazanavir was the most prescribed target antiretroviral and tipranavir the least prescribed within the first year after FDA approval. The peak number of

new prescriptions occurred within the first year after FDA approval for all the medications except darunavir, for which the number of prescriptions continued to rise. All three medications were initially prescribed almost exclusively to antiretroviral-experienced patients. Thus, the early peak uptake probably represents those highly antiretroviral-experienced patients for whom no or limited treatment options existed and who were awaiting the availability of new agents. In addition, an early benefit attributed to atazanavir over other available protease inhibitors or efavirenz was its favourable effect on lipids [14,15]. Thus, some of the early peak uptake in treatment-experienced patients may have occurred in those experiencing significant hyperlipidaemia on other protease inhibitor regimens. After the initial surge of veterans beginning treatment, uptake slowed but remained steady, a trend consistent with what has been reported by others when examining initiation of highly active antiretroviral therapy [16]. Variation in uptake among the targeted antiretrovirals occurred over time.

, 2011) Luria–Bertani (LB) broth was used as the basic culture m

, 2011). Luria–Bertani (LB) broth was used as the basic culture medium. Cells were precultured at 37 °C overnight with shaking (180 r.p.m.; BR-15: TAITEC, Tokyo, Japan). This culture (50 μL) was inoculated into 5 mL of LB at 37 °C with shaking (180 r.p.m.; BR-15). Logarithmic-phase cells were collected at an OD600 of 0.3. Cells from an overnight culture were harvested 15 h after inoculation from a glycerol

stock. To inhibit transcription/translation, cells were treated learn more with 100 μg mL−1 rifampicin and 100 μg mL−1 chloramphenicol for 60 min prior to harvesting. Cells equivalent to 8 × 108 colony-forming units (CFU) were collected at the logarithmic or stationary phase, washed with PBS and suspended in high osmotic or acid solutions. The high osmotic solutions were 4 M NaCl, 4 M KCl and 20% raffinose. The acid solutions were 10 mM HCl (pH 2.0) and citrate-phosphate buffer (pH 2.6, 4.6 or 6.6) supplemented with 100 mM NaCl and 10 mM KCl. After incubation for 5, 15 or 60 min, the cells were washed with PBS and collected for subsequent viability testing (CFU counting) and thin-layer chromatography (TLC). For heat- or cold-shock treatment, cultures containing 8 × 108 CFU signaling pathway were directly shifted to the

appropriate temperature. Lipid extraction and TLC were carried out as described previously (Tsai et al., 2011). Cells equivalent to 8 × 108 CFU were washed with PBS and resuspended in 200 μL of 2% NaCl. Lysostaphin was added to the cell suspension 2-hydroxyphytanoyl-CoA lyase (final concentration 0.1 mg mL−1) and incubated at 37 °C for 3 min. The lysed cell suspension was then subjected to chloroform–methanol extraction. Lipids were dissolved in chloroform–methanol (2 : 1;

v/v), applied to silica TLC plates (Silica gel 60; Merck, Darmstadt, Germany) and developed with chloroform–methanol–acetic acid (65 : 25 : 10; v/v/v). The TLC plates were sprayed with 100 mg mL−1 CuSO4 containing 8% phosphoric acid and heated at 180 °C to detect phospholipids. A digital image was obtained by a scanner, and the signal intensities were quantified using Image J software (version 1.44p; NIH). The number of CL synthase genes varies among bacterial species (Supporting Information, Table S1). Staphylococcal cls1 (SA1155) and cls2 (SA1891) share higher levels of similarity with each other than with cls genes from other species. They were grouped with Bacillus subtilis cls (BSU36590) and Listeria monocytogenes lmo2503, but not with B. subtilis ywjE (BSU37190) and ywiE (BSU37240) or L. monocytogenes lmo0008 (Fig. S1). This indicates that the two staphylococcal cls genes were not acquired by horizontal gene transfer from different species. We found a single insertion/deletion (INDEL) site in the N-terminal region of Cls (Fig. S1). The INDEL in Cls2 is considered to be the ancestral type because it is shared with the Cls of other bacterial species.

1 The WHO European Region has been free of autochthonous polio ca

1 The WHO European Region has been free of autochthonous polio cases from 1998, and certification by the WHO in 2002 of this condition led to a modification in vaccine administration. The oral polio vaccine (OPV) has gradually been replaced with the enhanced potency inactivated polio vaccine (IPV), safer than NVP-BGJ398 OPV because it is not associated with the rare

risk of vaccine-associated paralytic poliomyelitis (VAPP).3 In Italy, there is an increased and continuous inflow of refugees from countries where poliomyelitis is still present and this may represent a risk of the wild poliovirus strains being introduced. The Italian region of Puglia (Southern Italy) can be deemed a “border region” because, due to its geographic position, it has to face daily arrivals of refugees. The aim of this study was to evaluate the poliomyelitis immunization level, by titration check details of the neutralizing antibody, in a sample of refugees of various nationalities

residing in the Asylum Seeker Center in Bari Palese in Puglia. The study was carried out during 2008 and involved 573 refugees, 520 (90.8%; 95% CI = 88–92.9) males and 53 (9.2%; 95% CI = 7.1 − 12) females. Of these, 546 (95.3%; 95% CI = 93.1 − 96.8) were from Africa and 27 (4.7%; 95% CI = 3.2 − 6.9) from Asia. In particular, 20 residents (3.5%; 95% CI = 2.2 − 5.4) were from Afghanistan and 67 (11.7%; 95% CI = 9.2 − 14.7) from Nigeria. The average age of the population sample was 24.3 (SD = 5.4; range 1–50). Signed informed consent to the study was Staurosporine obtained from each participant. A 10 mL blood sample was obtained by venipuncture and the serum was separated by centrifugation. Each serum sample was coded and stored at −20°C. The immunity against poliomyelitis was evaluated as described previously.4 Demographic data from the Asylum Center database and the laboratory exam results were analyzed with the statistical software Epi-Info 6.00. Fisher’s exact test was used in the analysis of the difference between proportions. A value of p < 0.05 was considered as significant. An

antibody titer ≥1:8 was found in 571 subjects (99.6%) for poliovirus type 1, in 572 subjects (99.8%) for poliovirus type 2, and in 570 subjects (99.5%) for poliovirus type 3 (Table 1). All the subjects with an antibody titer less than 1:8 were males from Africa: specifically, a 20-year-old Nigerian with antibody titer less than 1:4 for the three types of poliovirus; two Somalis, aged 26 and 20, had antibody titers of 1:4 and 1:8, respectively. The levels of antibody titer did not significantly differ between Africans and Asians (Table 1). Our survey results revealed excellent immunization levels in the immigrants, in line with other studies in Europe in the last 15 years.5,6 However, we cannot exclude the existence of low-immunity pockets in the immigrant population just because they were not detected in our study.

Most literature focuses on

Most literature focuses on selleck inhibitor exploring pharmacists’ views and opinions of specific ethical dilemmas1 rather

than the decision-making process itself. Others have investigated factors influencing clinical decisions such as the sale of over-the-counter medication2. The aim of this study is to investigate the decision-making process of pharmacists and the factors influencing this process. Semi-structured qualitative interviews were used to identify the views of sixteen community pharmacists from a variety of backgrounds during February and March 2013. The average interview lasted for 33 minutes (range 9–90 minutes), and aimed to understand how pharmacists made decisions using a set of three practice-based hypothetical scenarios: supply of EHC to a minor, a confidentiality dilemma and a serious prescribing error. Interviews were audio recorded, transcribed verbatim, and thematically analysed. The study was given ethical approval by a senior academic in the University of Nottingham, Division of Social Research in Medicines

and Health. Pharmacists reported a number of different methods to make decisions. Some reported starting by considering relevant facts and then progressed to a decision. Pharmacist 5 reported ‘but it’s usually a question of looking at Ku-0059436 ic50 the facts, if it’s a professional decision thinking about the ethics, the legislation, the regulations, commercial aspects so basically put it all into a cooking pot …’ Others reported they made decisions by developing a range of options and then evaluating potential consequences allowing them to choose the least-worst option, ‘first of all I think about all the different options available … I try to put the patient first, but my main criteria is Rucaparib “would it get me into trouble”.’ (P16) Acting in the patients’ best interests was the most common theme regarding

influencing factors. Others included personal views and relationships with both patients and other healthcare professionals. One pharmacist said, ‘… but the focus … is always putting the patient first, making decisions in the best interests of the patient … taking on board all the information that I have …’ (P15). Another commented on their relationship with their GP, ‘I think it does affect my decision making because I like to make life easy for my GPs, because in making life easy for my GPs they respect me more and rely on me more and appreciate me more, … when I’m thinking about how to resolve problems I also think well what would my GPs like me to do, how do I make it easy for them and the patient’ (P8). Previous experiences were also reported as important, ‘It’s usually based on previous experience with regard to how that situation fits in initially with the law, with the code of ethics and patient’s needs …’ (P6). This study suggests that pharmacists employ a range of methods to make decisions.

Most literature focuses on

Most literature focuses on selleck chemicals llc exploring pharmacists’ views and opinions of specific ethical dilemmas1 rather

than the decision-making process itself. Others have investigated factors influencing clinical decisions such as the sale of over-the-counter medication2. The aim of this study is to investigate the decision-making process of pharmacists and the factors influencing this process. Semi-structured qualitative interviews were used to identify the views of sixteen community pharmacists from a variety of backgrounds during February and March 2013. The average interview lasted for 33 minutes (range 9–90 minutes), and aimed to understand how pharmacists made decisions using a set of three practice-based hypothetical scenarios: supply of EHC to a minor, a confidentiality dilemma and a serious prescribing error. Interviews were audio recorded, transcribed verbatim, and thematically analysed. The study was given ethical approval by a senior academic in the University of Nottingham, Division of Social Research in Medicines

and Health. Pharmacists reported a number of different methods to make decisions. Some reported starting by considering relevant facts and then progressed to a decision. Pharmacist 5 reported ‘but it’s usually a question of looking at Maraviroc price the facts, if it’s a professional decision thinking about the ethics, the legislation, the regulations, commercial aspects so basically put it all into a cooking pot …’ Others reported they made decisions by developing a range of options and then evaluating potential consequences allowing them to choose the least-worst option, ‘first of all I think about all the different options available … I try to put the patient first, but my main criteria is Rucaparib supplier “would it get me into trouble”.’ (P16) Acting in the patients’ best interests was the most common theme regarding

influencing factors. Others included personal views and relationships with both patients and other healthcare professionals. One pharmacist said, ‘… but the focus … is always putting the patient first, making decisions in the best interests of the patient … taking on board all the information that I have …’ (P15). Another commented on their relationship with their GP, ‘I think it does affect my decision making because I like to make life easy for my GPs, because in making life easy for my GPs they respect me more and rely on me more and appreciate me more, … when I’m thinking about how to resolve problems I also think well what would my GPs like me to do, how do I make it easy for them and the patient’ (P8). Previous experiences were also reported as important, ‘It’s usually based on previous experience with regard to how that situation fits in initially with the law, with the code of ethics and patient’s needs …’ (P6). This study suggests that pharmacists employ a range of methods to make decisions.

bethesdensis (AY788950), A cryptum (D30773), Swaminathania salit

bethesdensis (AY788950), A. cryptum (D30773), Swaminathania salitolerans (AB445099), Saccharibacter floricola (NR_024819), and Neoasaia chiangmaiensis (AB208549), were obtained from the NCBI website at http://www.ncbi.nlm.nih.gov/. To construct the phylogenetic tree of AAB, these 37 sequences were collected and nucleotide sequence alignment was carried out using clustalw (Larkin et al., 2007). We Cell Cycle inhibitor used the mega version 4.0 package

to generate phylogenetic trees to study the phylogenetic relationship based on 16S rRNA gene with the neighbor-joining (NJ) approach and 1000 bootstrap replicates (Tamura et al., 2007). Three hundred and ninety-one unique complete microbial genome sequences (one genome per genus) were obtained from the NCBI FTP website at ftp://ftp.ncbi.nlm.nih.gov/genomes/Bacteria/. Only amino acid-coding sequences on the chromosomes were used

for comparative analysis. For a homology search, a dataset of all proteins was constructed. The dataset of all proteins was constructed from all amino acid sequences from 391 complete microbial genomes. Four hundred and forty-three proteins on the KEGG metabolic map of G. oxydans were used as a query for the blastp homology search against the dataset of all proteins (Altschul et al., 1997; Kanehisa, 1997; Ogata et al., 1999; Kanehisa & Goto, 2000; LY2835219 Kanehisa et al., 2002, 2004, 2006, 2008, 2010). Of the 443 proteins, 293 were selected for further analysis because these ORFs exist in all three genera, Gluconobacter, Gluconacetobacter, and Acetobacter. Each homolog was identified by a homology search of amino acid sequence using the blastp filtering

expectation value of e-value ≤10−10 and sequence overlap ≥70% (Altschul et al., 1997). The top 50 hits were collected and multifasta files were created for phylogenetic analysis using house-written ruby scripts. The previously published complete genome sequences MRIP of Acetobactericeae, G. oxydans, G. diazotrophicus, A. pasteurianus, G. bethesdensis, and A. cryptum were obtained from the NCBI FTP website at ftp://ftp.ncbi.nih.gov/genomes/Bacteria/ (Prust et al., 2005; Greenberg et al., 2007; Azuma et al., 2009; Bertalan et al., 2009). Only protein-coding genes on the chromosomes were used for the identification of orthologous groups. Each orthologous gene was identified by homology searches for amino acid sequence using the blastp filtering expectation value of e-value ≤10−10 and sequence overlap ≥70% (Altschul et al., 1997). All ORFs were searched against each species, and the reciprocal best hits were regarded as being orthologous genes. If genes were orthologous among all species present, the group was defined as a unique orthologous dataset.

memory CD4 T cells Moreover, at different times after HIV infect

memory CD4 T cells. Moreover, at different times after HIV infection, Th17 cells recirculate in response to homeostatic drain, and may show different levels depending on the patient’s phase of infection at the time of selection [14]. Apart from infectious and autoimmune diseases, IL-17A has been shown to be associated with obesity and adipocyte development, indicating that IL-17A may mediate many interactions between adipose tissue and the immune system [2]. Our study is the first

report on IL-17A levels in HIV-1-infected subjects with and without central obesity, and shows that IL-17A levels are negatively related to visceral adipose tissue thickness. This result suggests a suppressive role of this cytokine in adipose tissue development. Conversely, a recent study by Sumarac-Dumanovic et al. showed that serum IL-17A is up-regulated in obese human patients and that obesity is positively correlated with enhanced selleck IL-17A expression and independent of other inflammatory factors [15]. A comparison between our results and those of Sumarac-Dumanovic et al. is complex for various reasons. First, most www.selleckchem.com/products/Fulvestrant.html of our study population were male, whereas the study by Sumarac-Dumanovic

et al. included only female subjects. Secondly, obesity was defined using different methods in the two studies. We assessed central obesity by measuring visceral fat thickness, whereas Sumarac-Dumanovic et al. used anthropometric indices [15]. The utility of sonography has been demonstrated based on its ability to evaluate intra-abdominal fat levels [16]. It has several advantages, such as simplicity, rapidity, availability,

safety and low cost, when compared with other techniques [8, 17]. Although unexpected, our results are consistent with recent data showing an anti-adipogenic role for IL-17A. It was Cyclin-dependent kinase 3 found recently that serum levels of IL-7 were decreased in obese subjects with metabolic syndrome [18]. The authors hypothesized a down-regulation of IL-17 by high levels of transforming growth factor (TGF)-β in subjects with metabolic syndrome [18]. IL-17A could delay the development of obesity and inhibit adipogenesis and fat development, as shown in murine models [5, 19]. Currently, the limited data on IL-17 are obtained with different methods. ELISA and flow cytometry are the main methods used for quantitating secreted cytokines, but the results are not directly comparable. The ELISA assay permits measurement of bulk cytokine secretion but does not necessarily reflect the expression of specific T-cell subsets (CD4, CD8, NK T and γδ T cells) [20]. Evaluation of other members of the IL-17 family and regulatory molecules of IL-17A (i.e. IL-6, IL-1β, TGF-β and IL-23) may clarify the biochemical process involved in the interaction between the immune system and somatic tissue. This was a cross-sectional study, and no conclusion regarding a causal relationship between IL-17A and visceral obesity can be made.