In this work, we propose the use of electrical parameters, capacitance and resistance of mangosteens, to determine the defect. The data are then analyzed employing logistic regression analysis to predict the probability for classification groups; in this case, those with and without translucent defect. The result from the logistic regression analysis reveals that the overall correctness of the prediction at 0.5 cut point is 87.3% which is competitive to those obtained by other means.”
“With a genome size of similar to 580 kb and approximately 480 protein coding regions, Mycoplasma genitalium is one of the smallest known self-replicating NSC 707544 organisms and, additionally,
has extremely fastidious nutrient requirements. The reduced genomic content of M. genitalium has led researchers to suggest that the molecular assembly contained in this organism may be a close approximation to the minimal set of genes required for bacterial growth. Here, we introduce a systematic approach for the construction and curation of a genome-scale in silico metabolic model for M. genitalium. Key challenges
included estimation of biomass composition, handling of enzymes with broad specificities, and the lack of a defined medium. Computational tools were subsequently employed to identify HTS assay and resolve connectivity gaps in the model as well as growth prediction inconsistencies with gene essentiality experimental data. The curated model, M. genitalium iPS189 (262 reactions, 274 metabolites), is 87% accurate in recapitulating in vivo gene essentiality results for M. genitalium. Approaches and tools described herein provide a roadmap for the automated construction of in silico metabolic models of other organisms.”
“cDNA fragments of lea3 genes with a high GC content (from
68 to 77%) were found in several Poaceae, including Sorghum vulgare, Saccharum officinarum, Oryza officinalis, Oryza meyeriana, Ampelocalamus calcareus, Cynodon dactylon, and Zizania latifoli. They were successfully isolated by means of optimal experimental parameters, which included dimethyl sulfoxide as additive and degenerate primers “”AGETKAS”" and “”AGKDKTG”", and their sequences were analyzed. Compared to the method BIX 01294 in vivo of isolating genes by screening of a cDNA library using abscisic acid- and other stress-responsive cDNA clones, which is time-consuming and costly, this method is relatively easy and inexpensive. Using this new method, many new homologue lea3 genes were rapidly determined.”
“A method to fabricate tubular nanoporous alumina layers by anodization of aluminum at current densities up to 1400 mA/cm(2) and anodization rates up to 70 mu m/min has been developed. It implies anodization in the meniscal region of the sample dipping into an electrolyte. The formed porous alumina has been found to be selforganized nanotube cells when the anodization current excides 100 mA/cm(2).